Abstract

The precise manipulation, quick cultivation, and delicate detection of yeast and bacteria are crucial in microbiology and biomedicine for behavior monitoring, identification of phenotypes, physiological assessment, and molecular analysis.

Microfluidic droplets have proven to be an effective method for encapsulating yeast and bacteria in highly monodisperse droplets for high-throughput screening and analysis of their phenotypes, subcellular structures, genes, and metabolites.This application note details the encapsulation of the yeast strain S. cerevisiae CEN.PK 113-7D and the bacterial strain L. cremorsi MG1363_GFP in double emulsions of 42 µm size using the Cell Encapsulation Platform, resulting in Microencapsulation of Bacteria and Yeast.